
Thermo Scientific Varioskan ALF Multimode Microplate Reader (VA000010C)
| Specification | Details |
|---|---|
| Absorbance Range | 200 to 1000 nm |
| Accuracy (Photometric) | ±1.0% + 0.003 Abs (0 to 2 Abs), ±2.0% (2.0 to 2.5 Abs) at 450 nm (96-well plate) |
| Bandwidth | Photometry: 2.5 nm |
| Depth (Metric) | 61 cm |
| Dimensions (L x W x H) | 61 x 34 x 40 cm |
| Dynamic Range | Fluorometry and Luminometry: ≥6 Decades |
| Emission Wavelength Range | 210-720 nm |
| Excitation Wavelength Range | 200-710 nm |
| Filters | Three factory installed fluorometric filter pairs: Coumarin family (Excitation 345/25 nm, Emission 450/40 nm); Fluorescein family (Excitation 485/15 nm, Emission 525/15 nm); Resorufin family (Excitation 555/15 nm, Emission 615/45 nm) |
| For Use With (Application) | Photometric, fluorometric and luminometric microplate assays |
| Height (Metric) | 40 cm |
| Includes | SkanIt software, Research Edition |
| Linearity | 0 to 2.5 Abs, 2.0% at 450 nm (96-well plate) |
| Measurement Speed | Photometry: ≤10 sec. (fast mode); Fluorometry and Luminometry: ≤20 sec. (96-well plate) |
| Precision | SD <0.003 Abs, or CV <1.0% at 450 nm |
| Product Type | Multimode Microplate Reader |
| Quantity | Microplate Reader + 1 year warranty |
| Sensitivity | Photometry (DNA): <1 ng/μL (96-well half area plate); Turbidimetry (formazin): <5 turbidity units (FTUs) (96-well plate); Fluorometry (fluorescein): 0.35 fmol/well (black 384-well plate); Luminometry (ATP): <275 amol/well (white 96-well plate) |
| Software Language Versions | English, French, German, Spanish, Portuguese, Italian, Japanese, Chinese |
| Spectral Scanning Speed | ≤16 sec. (Fast mode) |
| Voltage | 100/115/220/230 V |
| Warranty | 1-year Standard |
| Weight (Metric) | 24.5 kg |
| Width (Metric) | 34 cm |
| Cuvettes | No |
| Detector Type | Photomultiplier Tube (PMT) |
| Incubator Temperature | Ambient +2° to 45°C |
| Interface | PC Software (Thermo Scientific SkanIt Software) |
| Light Source | Xenon Flash Lamp |
| Product Line | Varioskan |
| Reading Options | Endpoint, Kinetic |
| Shaking | Linear, Orbital, Double Orbital |
| Software Type | SkanIt Software |
| Unit Size | 1 each |
Product Details
Enhance your fluorescence and luminescence measurements with the filter-based optics
With the Varioskan ALF reader, fluorescence and luminescence measurements are performed with filter-based optics for optimal specificity and sensitivity. The system comes with three pre-installed excitation and emission filter sets suitable for many common fluorometric applications like Quant-IT DNA quantitation assays and PrestoBlue and AlamarBlue cell viability assays. Additionally, five open filter positions are available for customized fluorometric and luminometric measurements. The filter slots are easy to access on the instrument's side with no tools required for filter exchange. Also, commonly used FRET and BRET assays are easy to set up and perform thanks to Varioskan ALF reader's unique filter-based optics design.
This package includes a Varioskan ALF Multimode Microplate Reader (Cat. No. VA000010C) and a Rapid Exchange two-year extended warranty.
Achieve consistently accurate results with automatic gain adjustment
The automatic gain adjustment feature selects the ideal fluorometric or luminometric reading range for your instrument based on signal intensity in the well, eliminating the need to manually adjust measurement parameters. The results are consistent no matter what signals are measured.
Additional features
- Kinetic and endpoint measurements for a variety of applications
- Spectral scanning for photometric measurements
- Separate optimized measurement modes for absorbance and turbidimetric measurements
- A self-diagnostics system that includes a set of initialization tests and adjustments, ensuring instrument readiness for operation
- Compatible with 6- to 384-well microplates with and without lids
- Reads μDrop and μDrop Duo plates for microvolume analysis of DNA, RNA, and proteins
- Performs onboard shaking and incubation for temperature-critical assays
- Operated through SkanIt software
- Unlimited installation on multiple computers, no annual fee required
- Includes an extensive cloud-based library of ready-made protocols
- Two editions available: Research Edition for life sciences and Drug Discovery Edition with features for compliance with 21 CFR Part 11
- Multiple user interface languages available
- Automation compatibility for integration with automation interface
- Thermo Scientific verification plates provide comprehensive and traceable validation of the instrument optical performance. Ready-made SkanIt software protocols provide easy to read reports with defined measurement parameter limits
Varioskan ALF Multimode Microplate Reader with 2-year Extended Warranty (A66711), REX is a Varioskan ALF specific package SKU including the instrument (VA000010C), and an extended warranty to 2 years which includes a rapid exchange service (REX) should the instrument get broken during the warranty period.

